Biochemistry & Genetics: PreTest Self-Assessment & Review by Golder Wilson

By Golder Wilson

Supplies clinical scholars 500 questions, solutions, and factors to organize for the biochemistry and genetics sections of the USMLE Step 1. the recent variation comprises many new questions within the two-step medical structure to simulate the USMLE Step 1.

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The sequence of codons that make up a gene exhibits an exact linear correspondence to the sequence of amino acids in the translated protein 34. Sickle cell anemia (141900) is caused by a point mutation in the hemoglobin gene, resulting in the substitution of a single amino acid in the β-globin peptides of hemoglobin. This mutation is best detected by which of the following? a. Isolation of DNA from red blood cells followed by polymerase chain reaction (PCR) amplification and restriction enzyme digestion b.

B. c. d. e. Golgi apparatus Smooth endoplasmic reticulum Free polysomes Nucleus Rough endoplasmic reticulum 56. Which of the following statements regarding eukaryotic cells is true? a. b. c. d. e. Formylated methionyl-tRNA is important for initiation of translation Single mRNAs specify more than one gene product Cycloheximide blocks elongation during translation Cytosolic ribosomes are smaller than those found in prokaryotes Erythromycin inhibits elongation during translation 57. Modification of mRNA so that a signal sequence is added to the amino terminus of the cytosolic protein, α-globin, results in a.

30. The answer is b. (Murray, pp 435–451. Scriver, pp 3–45. Sack, pp 3–29. ) Primase is a DNA-dependent RNA polymerase located in the primosome at the replication fork of DNA. Primase initiates DNA synthesis by synthesizing a 10-base RNA primer. The DNA-RNA helix formed binds DNA polymerase III, which synthesizes a DNA fragment (the Okazaki fragment) in a 5′ to 3′ direction. When the RNA primer of the previous Okazaki fragment is met, DNA polymerase I replaces III and digests the RNA primer, replacing it with appropriate DNA bases.

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