By Nadine G. Rouphael, David S. Stephens (auth.), Myron Christodoulides (eds.)
Neisseria meningitidis is a huge causative agent, world wide, of probably life-threatening meningitis and septicaemia, which hold with them a excessive mortality expense and everlasting actual and neurological concerns for survivors. Neisseria meningitidis: Advanced equipment and Protocols deals a suite of equipment and protocols that mirror the improvement and refinement of a number of new applied sciences utilized to the meningococcus as awarded by means of specialist researchers. numerous of the chapters during this booklet describe equipment that depend upon the gathering of entire units of organic facts, for instance, utilizing the genome to generate transcriptomes, proteomes, and metabolomes. Written within the hugely profitable equipment in Molecular Biology™ sequence structure, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, simply reproducible laboratory protocols, and pointers on troubleshooting and fending off recognized pitfalls.
Authoritative and state of the art, Neisseria meningitidis: Advanced equipment and Protocols may be effectively used to check different pathogens and illnesses and will have extensive entice scientific and non-clinical scientists alike; even though, there's nonetheless a lot to benefit concerning the meningococcus and its advanced nature, which in flip keeps to bare the most important information about human biology as well.
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Extra info for Neisseria meningitidis: Advanced Methods and Protocols
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Trotter CL, Ramsay ME (2007) Vaccination against meningococcal disease in Europe: review and recommendations for the use of conjugate vaccines. FEMS Microbiol Rev 31:101–7. Lepeyssonnie (1963) La méningite cérébrospinale en Afrique. Bull WHO 28:53–114. Molesworth AM, Cuevas LE, Connor SJ et al (2003) Environmental risk and meningitis epidemics in Africa. Emerg Infect Dis 9:1287–93. Greenwood BM, Bradley AK, Wall RA et al (1985) Meningococcal disease and season in sub-Saharan Africa. Lancet 2:829–30.
Mandrell RE, Zollinger WD (1977) Lipopolysaccharide serotyping of Neisseria meningitidis by hemagglutination inhibition. Infect Immun 16:471–5. Zughaier SM, Tzeng YL, Zimmer SM et al (2004) Neisseria meningitidis lipooligosaccharide structure-dependent activation of the macrophage CD14/Toll-like receptor 4 pathway. Infect Immun 72:371–80. Zughaier S, Steeghs L, van der Ley P et al (2007) TLR4-dependent adjuvant activity of Neisseria meningitidis lipid A. Vaccine 25:4401–9. Braun JM, Blackwell CC, Poxton IR et al (2002) Proinflammatory responses to lipooligosaccharide of Neisseria meningitidis immunotype strains in relation to virulence and disease.
1007/978-1-61779-346-2_2, © Springer Science+Business Media, LLC 2012 21 22 P. Brandtzaeg and M. van Deuren invasive disease. A limited number of meningococci belonging to specific clones or clonal complexes with serogroups A, B, C, Y, and W135 cause >95% of systemic meningococcal disease (SMD) (1). Encapsulated meningococci expressing pili, opacity proteins, and other subcapsular adhesion molecules are transferred by droplets or by direct contact (kissing) from an asymptomatic carrier to a nonimmune person.