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Bacillus pumilus and S. aureus, while fruit and root extracts in double strength gave positive results against a Gram-positive bacillus (B. subtilis). The leaf petroleum ether extract of C. colocynthis showed 100% mortality against A. aegypti and C. quinquefasciatus (Rahuman and Venkatesan 2008). The fatty acids, oleic acid and linoleic acid were isolated and identified in C. colocynthis petroleum ether extract. As mosquito larvicidal compounds oleic and linoleic acids were quite potent against fourth-instar larvae of A.
The n-hexane, carbon tetrachloride, and chloroform soluble fractions of the methanol extract of C. asiatica were subjected to antioxidant, antimicrobial and brine shrimp lethality bioassays (Obayed Ullah et al. 2009). The adulticidal and larvicidal effects of leaf hexane, chloroform, ethyl acetate, acetone, and methanol extracts of C. asiatica were investigated against the adult cattle tick H. bispinosa, sheep fluke P. cervi, fourth-instar larvae of the malaria vector A. subpictus and Japanese encephalitis vector C.
Quinquefasciatus (Rahuman et al. 2008b). Triterpenoid acids, volatile and fatty oils, alkaloids, glycosides, flavonoids, and steroids have been isolated from different parts of the plant (Jayashree et al. 2003). 11 Citrullus colocynthis Linn. (Cucurbitaceae) Is an annual herb found in the wild as well as cultivated throughout India in warm areas. It is locally known as Makkal in Hindi, bitter apple in English, and Paitummatti in Tamil. In traditional medicine, this plant has been utilized to treat constipation, diabetes, edema, fever, jaundice, bacterial infections as well as cancer.