Cell Separation. Methods and Selected Applications, Volume 3 by Thomas G., II Pretlow

By Thomas G., II Pretlow

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Keller, D . Kraft, and O. ), pp. 2 7 3 286. Pergamon, Oxford. , and Wagner, H. (1980). Alloreactive and H-2-restricted Lyt 23 cytotoxic L lymphocytes derive from a common pool of ante­ cedent Lyt 123 precursors. J. Exp. Med. 152, 1413-1418. Hellstrom, U . , and Perlmann, P. (1976a). Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to Sepharose beads. Scand. J. Immunol. 5 (Suppl. 5), 4 5 - 5 5 . Hellstrom, U . , and Perlmann, P. (1976b). Fractionation of human T lymphocytes on wheat germ agglutinin-Sepharose.

The P N A " thymocytes were shown to be respon­ sible for the production of a series of interleukins (lymphocyte stimulating factors that are not H-2 or species restricted) by mitogen-stimulated T cells (Basham et aL, 1981). , in presence of thymic epithelial supernatants, thymic hormones, or interleu+ + + + 27 2. LECTINS FOR S E P A R A T I O N OF CELLS kins) they acquired new cell m e m b r a n e markers characteristic of mature thymocytes, including the lack of the P N A receptor and the ability to re­ spond to P H A .

1982). , 1981). Binding of the lectin did not closely parallel the presence of cy­ totoxic activity, raising the possibility that the cytotoxic cells may only be a subset of the VVL binding lymphocytes. , 1981) that murine T cell blasts against H-2 or parasite antigens all had comparable levels of VLL binding, although the cytolytic activity of these cells varied widely. Furthermore, when lymphoblasts bind­ ing large or small a m o u n t s of VVL were sorted on the basis of their relative fluorescence intensity and tested for cytolytic activity, no appreciable dif­ ference in activity between the two populations was observed.

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