By Margaret I. Tyler (auth.), Catherine Cooper, Nicolle Packer, Keith Williams (eds.)
Amino acid research is customary in biotechnology, biomedical, and foodstuff research laboratories. Amino Acid research Protocols constitutes a big number of those fundamental analytical thoughts, either vintage and state of the art, of excessive software for answering particular organic questions. universal equipment comprise these in keeping with HPLC or fuel chromatography separation and research after precolumn derivatization. New thoughts in line with capillary electrophoresis separation, high-performance anion trade chromatography, and mass spectrometry also are provided. because effects count seriously at the caliber of the pattern, such a lot participants have dedicated a piece to pattern instruction, really to the gathering and garage of physically fluids. a brand new technique for desalting samples ahead of hydrolysis can be supplied. each one procedure is defined in step by step element to make sure winning experimental effects, and comprises necessary notes on pitfalls to prevent, and adaptations that let the how you can be used with various systems.
updated and hugely useful, Amino Acid research Protocols deals analytical and scientific chemists, in addition to a vast variety of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the effective research of either universal and unusual amino acids.
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Additional info for Amino Acid Analysis Protocols
Ling, unpublished data). 17. Most amino acids will be present in great abundance, saturating the detector, but the glycine and proline peaks are usually still on-scale, and thus can be used for mol Nle per mol protein quantitation. Chromatograms for 40 μg hydrolysates of a recombinant protein spiked with 0, 200, or 400 pmol Nle are given in Fig. 3A, B, and C, respectively. 18. Sensitive detection of hydroxylysine (Hyl) is difficult because of the fact that most noncollagenous molecules are at most partially modified at just one -Lys-Gly- positio n, thus the overall percentage of modified Lys is very low.
Academic, San Diego, CA, pp. 299– 306. 10. Cohen, S. A. and De Antonis, K. M. (1994) Applications of amino acid analysis derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate: Analysis of feed grains, intravenous solutions and glycoproteins. J. Chromatog. 661, 25–34. 11. Van Wandelen, C. and Cohen, S. A. (1997) Using quaternary high-performance liquid chromatography eluent systems for separating 6-aminoquinolyl-N- Precolumn Derivatization with AQC 47 hydroxysuccinimidyl carbamate-derivatized amino acid mixtures.
Merewether, L. , Clogston, C. , and Lu, H. S. (1994) Peptide map analysis of recombinant human granulocyte stimulating factor: elimination of methionine modification and nonspecific cleavages. Anal. Biochem. 216, 135–146. 18. Allen, G. (1989) Determination of the carboxy-terminal residue, in Sequencing of Proteins and Peptides, Elsevier, Amsterdam and New York, pp. 67–71. 19. Grunau, J. A. and Swaider, J. M. (1992) Chromatography of 99 amino acids and other ninhydrin-reactive compounds in the Pickering lithium gradient system.