By Juan Hong Juan A. Asenjo
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dependent and straightforward types of enzymatic lysis and disruption of yeast cells / J.B. Hunter and J.A. Asenjo --
twin hollow-fiber bioreactor for cardio whole-cell immobilization / Ho Nam Chang, Bong Hyun Chung, and In Ho Kim --
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Use of aqueous two-phase structures for restoration and purification in biotechnology / Bo Mattiasson and Rajni Kaul --
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Modeling of precipitation phenomena in protein restoration / C.E. Glatz and R.R. Fisher --
strategy concerns for scale-up of liquid chromatography and electrophoresis / S.R. Rudge and M.R. Ladisch --
Mathematical modeling of bioproduct adsorption utilizing immobilized affinity adsorbents / Somesh C. Nigam and Henry Y. Wang --
High-resolution, high-yield continuous-flow electrophoresis / William A. Gobie and Cornelius F. Ivory --
Scale-up of isoelectric focusing / Milan Bier --
Large-scale gel chromatography : overview of application for purification of protein items from microbial resources / James J. Kelley, George Y. Wang, and Henry Y. Wang --
Electron paramagnetic resonance spectroscopy reviews of immobilized monoclonal antibody constitution and serve as / Erik J. Fernandez, Forrest B. Fernandez, Roger B. Jagoda, and Douglas S. Clark.
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2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Rony, P. R. Biotechnol. Bioeng. 1971, 13, 431. Waterland, L. ; Michaels, A. ; Robertson, C. R. AIChE J. 1974, 20, 50. Knazek, R. ; Gullino, R. ; Kohler, P. ; Dedrick, R. L. Science, 1972, 178, 65. Kan, J. ; Shuler, M. L. Biotechnol. Bioeng. 1978, 20, 217. Vick Roy, T. ; Blanch, H. ; Wilke, C. R. Biotechnol. Lett. 1982, 4, 483. Shuler, M. L. Ann. Y. Acad. Sci. 1981, 369, 65. Inloes, D. ; Smith, W. ; Taylor, D. ; Cohen, S. ; Michaels, A. ; Robertson, C.
The present study reports the f i r s t successful long-term c u l t i v a t i o n of rifamycin-B producing Nocardia mediterranei and the problems encountered i n growing IS. c o l i and A_. niger c e l l s i n the dual hollowf i b e r bioreactor. Materials and Methods Materials and s t r a i n s . Yeast extract, bactopeptone, malt extract and trypton were products of Difco Laboratories and glucose was from Hayashi Pure Ind. (Tokyo, Japan). E. c o l i (Sigma EC-1, a l k a l i n e phosphatase-rich mutant) was from Sigma Chemical Co.
The volumetric productivity of the reactor was 125 pg/mL/h based on the void voulme of the reactor where the c e l l s were a c t u a l l y immobil i z e d . This was about t h i r t y - f o l d as compared to that of the flask culture. This number drops to 15 or 10 i f we include the reactor volume or the volume of the glass tubing used. This high p r o d u c t i v i ty comes essentially from a highly dense c e l l mass i n the reactor shown i n the electron micrograph ( F i g . 9). The measured dry c e l l mass was 600 g/L.