By G. Di Francia, P. Maddalena, I. Rendina, C. Di Natale, A. D'Amico
This ebook features a collection of papers provided on the twelfth Italian convention on Sensors and Microsystems. It presents a different point of view at the learn and improvement of sensors, microsystems and comparable applied sciences in Italy. The medical values of the papers additionally bargains a useful resource to analysts desiring to survey the Italian state of affairs approximately sensors and microsystems. In an interdisciplinary method, many points of the disciplines are lined, starting from fabrics technology, chemistry, utilized physics, digital engineering and biotechnologies.
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1 Experimental setup of the scanning mode. A laser beam formation SPR spectrometer "BioHelper". unit (dashed line) is placed on the angle scanning system. The unit consists of a semiconductor laser (A = 650 nm) and collimating optics (2). A concave cylindrical lens (that prevents the collimated laser beam from focusing on the convex surface of the main lens (1)) also is placed in the scanning system. A stepper motor maintains the scanning angle 0 at 23" (from a starting angle of 52"). 035". The gold nanoparticles with expected diameter of 20nm were produced by wellknown procedure [6] by citrate reduction form HAuC14 solution.
0" for investigating DNA interactions, we have performed real-time measurements for immobilization of probe, hybridization and regeneration. The data on figure 4 shows kinetic curves for immobilization of thiolated oligonucleotide (probe) on the clean gold. 0 angle minutes in both scattering and reflection channels. We have investigated the amplification effect of 20nm gold nanoparticles (GNP) on the oligonucleotide immobilization process. We found that the signal on probe immobilization can be greatly increased by using GNP.
In brief, a QCM consists of a thin quartz crystal sandwiched between a pair of golden electrodes. Due to the piezoelectric properties of quartz, it is possible to make resonant the crystal by applying an AC voltage across its electrodes. The resonant frequency of the crystal depends on the total oscillating mass. When a mass is adsorbed to the sensor crystal the frequency decreases and the oscillation frequency shift is proportional to the mass adsorbed onto the surface as stated by the Sauerbrey relation [2].