By David R. Edgell
Homing Endonucleases: tools and Protocols goals at delivering molecular biologists with a complete source to spot and signify homing endonucleases from genomic series, to infer the organic foundation of binding and cleavage specificity, in addition to to supply protocols to revamp endonuclease aim specificity for genome-editing purposes. Engineering of fashion designer homing endonucleases has set the level for genome modifying of complicated eukaryotic genomes with a large variety of capability functions together with specific gene knockouts in version organisms and gene treatment in people, making this booklet a invaluable source for destiny learn. Written within the hugely winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, quite simply reproducible laboratory protocols and pointers on troubleshooting and warding off identified pitfalls.
Authoritative and useful, Homing Endonucleases: tools and Protocols serves as a key reference for all labs learning site-specific DNA endonucleases.
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Steczkiewicz K, Muszewska A, Knizewski L, Rychlewski L, Ginalski K (2012) Sequence, 24 93. 94. 95. 96. 97. 98. 99. 100. 101. 102. 103. Marlene Belfort and Richard P. Bonocora structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily. Nucleic Acids Res 40:7016–7045 Rusch DB, Halpern AL, Sutton G, Heidelberg KB, Williamson S, Yooseph S, Wu D, Eisen JA, Hoffman JM, Remington K et al (2007) The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific.
Nat Biotechnol 31:223–239 Chapter 2 Bioinformatic Identification of Homing Endonucleases and Their Target Sites Eyal Privman Abstract Homing endonuclease genes (HEGs) are a large, phylogenetically diverse superfamily of enzymes with high specificity for especially long target sites. The public genomic sequence databases contain thousands of HEGs. This is a large and diverse arsenal of potential genome editing tools. To make use of this natural resource, one needs to identify candidate HEGs. Due to their special relationship with a host gene, it is also possible to predict their cognate target sequences.
In: Belfort M, Derbyshire V, Stoddard BL, Wood DW (eds) Homing endonucleases and inteins. Springer, Berlin, pp 161–175 21. Pietrokovski S (1994) Conserved sequence features of inteins (protein introns) and their use in identifying new inteins and related proteins. Protein Sci 3:2340–2350 22. Gibb EA, Edgell DR (2010) Better late than never: delayed translation of intron-encoded endonuclease I-TevI is required for efficient splicing of its host group I intron. Mol Microbiol 78:35–46 23. Stoddard BL, Belfort M (2010) Social networking between mobile introns and their host genes.