Genetics and Biotechnology of Lactic Acid Bacteria by M. J. Gasson, G. F. Fitzgerald (auth.), Michael J. Gasson,

By M. J. Gasson, G. F. Fitzgerald (auth.), Michael J. Gasson, Willem M. De Vos (eds.)

A best reference quantity for geneticists, nutrition technologists and biotechnologists within the educational and business sectors. Fermentations with lactic acid micro organism be certain vital characteristics resembling style, shelf-life, and foodstuff values. New tools of nutrients creation require speedy and trustworthy manufacture, which has ended in a dramatic surge of curiosity within the genetic, microbiological and biochemical houses of lactic acid bacteria.

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Extra resources for Genetics and Biotechnology of Lactic Acid Bacteria

Sample text

Transposon Tn916 has been the subject of detailed molecular analysis and there is a very good understanding of its mode of transposition. Recent work using L. lactis subsp lactis MG 1363 has generated interesting data that are relevant to the exploitation of this transposon. Bringel et al. (1991) showed that both Tn916 and Tn919 retain the same chromosomal location following their transfer between strains of the L. lactis C2/712 family. Furthermore this transfer still took place when a mutant transposon Tn916-intl was used.

Transposons Tn916 and Tn919 are similar conjugative elements that were originally isolated in Enterococcus faecalis DS 16 (Franke and Clewell, 1981) and Streptococcus sanguis FCI (Fitzgerald and Clewell, 1985), respectively. It is well established that these elements promote their own transfer to a wide variety of different Gram-positive strains and in many cases they integrate randomly into the host strain's genome. Hill et al. (1985) first demonstrated that Tn919 could be transferred into lactic acid bacteria from E.

Coli Hfr strain. This possibility is also suggested by the observation of conjugal transfer of integration defective Tn916 transposons in the same strain, as reported by Bringel et al. 2. 2 Recombination genes A derivative of L. lactis subsp. lactis ML3 that was deficient in generalized recombination was isolated by Anderson and McKay (1983) using ethyl methane sulphonate mutagenesis and a screen for methyl methane sulphonate and UV sensitive survivors indicative of a defective SOS repair system.

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