By R. H. Davis (auth.), Professor Dr. Ulrich Kück (eds.)
Since booklet of the 1st version of quantity II in 1995, a number of advancements in fungal molecular biology - corresponding to fungal genome initiatives - have improved enormously. This in flip has affected primary genetics in addition to biotechnology. to deal with those advancements, the second one version has been thoroughly up to date and all chapters were revised. additionally, the amount includes 5 new chapters facing diverse facets of fungal molecular genetics.
Topics contain: Nuclear and extranuclear genetics; practical genomics; biotechnical genetics; yeasts and filamentous fungi.
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Sample text
Mitotic Mapping ..................... C. Comparison of Meiotic and Mitotic Analysis . . . . . . . . . . . V. Strategies and Protocols . . . . . . . . . A. Growth Conditions and Strains ............ 1. Media and Growth Conditions .......... 2. Induction of Mutants . . . . . . . . . B. Methods for Genetic Analysis ............. 1. Sexual Crosses ....................... 2. Heterokaryons ....................... 3. Isolation of Diploids .................. 4. Haploidization .
This explains the difficulty of finding mutants of either one. One of the systems (represented by mus-38, in a distinct complementation group) is probably a component of the classical nucleotide excision repair (NER) system characterized well in E. coli, and was found by search for a homologue of the S. cerevisiae RADi gene. The second system (UV damage excision repair, or UVDE) is represented by a single mutant, mus-i8, and accomplishes dimer and photoproduct excision by way of an endonuclease with different specificity.
Sometimes complementation tests must be performed with heterozygous diploids as some media do not allow heterokaryosis. For example, this is the case with acetate (Armitt et al. 1976) and with benzoate (Boschloo et al. 1990). , glycerol nonutilising mutants), but in contrast they do in heterozygous diploids. The development of techniques for gene replacement by transformation (Miller et al. 1985) also opened possibilities for the isolation of strains with disrupted genes resulting in a mutant phenotype.